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The effects of solvent viscosity about the catalytic fee constants with the hydrolysis of 1 by Zn-NagA are proven in Figures 4A and 4B employing sucrose because the micro-viscogen. The plot of relative k

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enzyme) within the adjacent subunit ion-pairs Together with the phosphate moiety on the substrate (ten). It can be unlikely that this arginine residue could lead to your diminution in k

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Inside the crystal construction from the D273N mutant of NagA, the aspect chain of His-143 is three.0 Å faraway from the phosphonate oxygen on the mimic on the tetrahedral intermediate (21). This outcome indicates that this group could aid the activation on the carbonyl group in the substrate along side an interaction with the metallic ion sure inside the Energetic web-site.

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